New EuroClonality publication on 1-tube TRG assays, discontinuation of commercial TRG 2-tube assay
Production and sales of the EuroClonality/BIOMED-2 TRG tube A-B assays has been discontinued by Invivoscribe, as the Invivoscribe TRG2.0 assay (1-tube assay) is available since a couple of years. The Invivoscribe TRG2.0 assay performance has recently been evaluated in a multi-center study in comparison with the EuroClonality/BIOMED-2 TRG 2-tube assay as well as a new EuroClonality TRG 1-tube assay (Armand et al, HemaSphere 2019;3:e255). The latter was designed with the following specifications: (i) to regroup PCR products within a limited size range, (ii) to generate relatively short PCR products (<200 bp), (iii) to combine all primers within a single tube. Compared to the classical EuroClonality/BIOMED-2 TRG 2-tube assay, both 1-tube assays displayed fairly similar performances and demonstrated easier interpretation as well as a lower risk of false positives from minor peaks in dispersed repertoires. In addition, both assays generate smaller fragments, likely to be better adapted to analysis of formalin-fixed paraffin-embedded tissue samples.
New EuroClonality-NGS publications and SOPs
Three new manuscripts of the EuroClonality-NGS Working Group have been accepted in Leukemia. Two SOPs related to these publications are available in the protocols section of the EuroClonality website: “NGS-based clonality assessment of immunoglobulin gene rearrangements for the Ion Torrent platform” and “Two-step IG/TR NGS-based marker identification for the Illumina MiSeq platform”.
EQA 2019 registration
Registrations for the 2019 External Quality Assessment Scheme for IG/TR Clonality Testing in Suspected Lymphoproliferations/Lymphoma organized by the EuroClonality network, is open.
EuroClonality-NGS Consortium on Next-Generation Sequencing for IG / TR immunogenetic analysis
Workshop 2021: February 8-10
Nijmegen, the Netherlands
Ig/TCR clonality interpretation and reporting tables in different languages.
When using this Tables, please refer to www.euroclonality.org and to the original publication in which the English version of the Tables is published: Langerak, et all., Leukemia 2012;26:2159-2172
Dr. Patricia Groenen, chair
Dr. Anton Langerak, treasurer
Prof. Dr. Frederic Davi, secretariat
Dr. Jose Cabeçadas
Dr. Paula Gameiro
Prof. Jacques J.M. van Dongen, founding chairman 1996-2011
Project manager EuroClonality